• 说明书Z03470-1-eSpCas9-N-NLS Nuclease.pdf

产品信息

产品描述

    eSpCas9 nuclease is a mutant form of Cas9 nuclease. Cas9 nuclease is an RNA-guided endonuclease that can catalyze cleavage of double stranded DNA. This kind of targeted nuclease is a powerful tool for genome editing with high precision. Cas9 protein forms a very stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component of the CRISPR/Cas9system. Compared with the wild type Cas9 nuclease, eSpCas9 reduces off-target effects by over 10-fold, while maintaining robust on-target genome editing efficiency. We has developed a eSpCas9-N-NLS nuclease which contains a nuclear localization sequence (NLS) on the N-terminus of the protein to meet all the researchers’ requirements (e.g. in vitro cleavage assay, RNP complex transfection, and micro injection).

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